Mouse Glucose Transporter 4 (GLUT4) ELISA Kit

Cat.#:

JL20505

Regulatory Status:

RUO

Alternative:

SLC2A4; Solute carrier family 2; facilitated glucose transporter member 4; Glucose transporter type 4; insulin-responsive

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.082ng/Ml

Specificity:

This kit recognizes Mouse GLUT4 in samples. No significant cross-reactivity or interference between Mouse GLUT4 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates;cell culture supernatants and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GLUT4

Glucose transporter type 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4, is a protein encoded by the SLC2A4 gene. GLUT4 is the insulin-regulated glucose transporter found primarily in adipose tissues and striated muscle. At the cell surface, GLUT4 permits the facilitated diffusion of circulating glucose down its concentration gradient into muscle and fat cells. Once within cells, glucose is rapidly phosphorylated by glucokinase in the liver and hexokinase in other tissues to form glucose-6-phosphate, which then enters glycolysis or is polymerized into glycogen.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse GLUT4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-102	97
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse GLUT4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-94	90-110
1 : 2	Average (%)	90	96
1 : 4	Range (%)	89-103	105-115
1 : 4	Average (%)	94	108